Journal: Oncology Reports

Article Title: TRIM46 deficiency-induced DNA damage enhances the sensitivity of cisplatin in non-small cell lung cancer by regulating the Akt signaling pathway

doi: 10.3892/or.2026.9063

Figure Lengend Snippet: TRIM46 depletion inhibits cell proliferation by regulating the Akt signaling pathway. (A) Cell proliferation of A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (B) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 expression in A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (C) Cell proliferation of A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. (D) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 in A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. ***P<0.001 vs. shNC or Vector + Vehicle; ### P<0.001 vs. TRIM46 + Vehicle. TRIM46, tripartite motif 46; DDP, cisplatin; sh, short hairpin; NC, negative control; OD, optical density; p-, phosphorylated.

Article Snippet: The anti-Akt (cat. no. 9272; 1:1,000 for western blotting), anti-phosphorylated (p-)Akt (cat. no. 4060; 1:1,000 for western blotting) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies (cat. no. 5174; 1:3,000 for western blotting) were purchased from Cell Signaling Technology, Inc. 3,3′-Diaminobenzidine (DAB) detection kit (cat. no. 34002), Roswell Park Memorial Institute 1640 medium (RPMI-1640; cat. no. 11875101) and fetal bovine serum (FBS; cat. no. 10099141C) were purchased from Gibco (Thermo Fisher Scientific, Inc.).

Techniques: Transduction, Knockdown, Western Blot, Expressing, Over Expression, Plasmid Preparation, Negative Control

Journal: Oncology Reports

Article Title: TRIM46 deficiency-induced DNA damage enhances the sensitivity of cisplatin in non-small cell lung cancer by regulating the Akt signaling pathway

doi: 10.3892/or.2026.9063

Figure Lengend Snippet: Mechanism of TRIM46 deficiency-induced DNA damage, enhancing the sensitivity of DDP in NSCLC by regulating Akt signaling pathway. (A) TRIM46 expression was positively associated with DDP resistance in NSCLC tissues. (B) TRIM46 overexpression significantly suppressed DDP-induced apoptosis and enhanced DDP resistance in A549 cells. (C) TRIM46 knockdown induced DNA damage by modulating the protein levels of p-AKT, RAD51, caspase 3, and cleaved-caspase 3, thereby resulting in cell proliferation inhibition in NSCLC cells. (D) TRIM46 knockdown increased the sensitivity of xenograft tumors to DDP treatment. NSCLC, non-small cell lung cancer; TRIM46, tripartite motif 46; DDP, cisplatin; p-, phosphorylated.

Article Snippet: The anti-Akt (cat. no. 9272; 1:1,000 for western blotting), anti-phosphorylated (p-)Akt (cat. no. 4060; 1:1,000 for western blotting) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies (cat. no. 5174; 1:3,000 for western blotting) were purchased from Cell Signaling Technology, Inc. 3,3′-Diaminobenzidine (DAB) detection kit (cat. no. 34002), Roswell Park Memorial Institute 1640 medium (RPMI-1640; cat. no. 11875101) and fetal bovine serum (FBS; cat. no. 10099141C) were purchased from Gibco (Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Over Expression, Knockdown, Inhibition